Effect of adoptively transferredAspergillus-pulsed DCs on resistance to aspergillosis or candidiasis. Splenic DCs were pulsed with either viable Aspergillus conidia or hyphae for 2 hours at 37°C before the addition of amphotericin B to prevent fungal overgrowth or transfected with fungal RNA for 2 hours at 37°C before wash. DCs (5 × 105) were administered into recipient mice subcutaneously, twice, 2 weeks and 1 week before the intravenous injection of 5 × 105A fumigatus conidia (A) or C albicans (B). Resistance to infection was assessed in terms of median survival time (MST; days) and CFUs, mean ± SE. *P < .05 (mice receiving pulsed DCs versus mice not receiving DCs or receiving unpulsed DCs). In parentheses are the number of dead animals over total injected.
