TLR agonist-mediated effects on phagocytosis. Opsonized latex beads were incubated with prestimulated neutrophils at a ratio of 25:1 for 30 minutes at 37°C. Phagocytosis was measured by flow cytometry (A) and microscopy (B). Data are presented as percent of cells in the bead-positive gate (A) or number of internalized particles per 100 cells × 100 (B). *P < .01, as determined by Student t test, compared with untreated cells. Error bars indicate standard deviation of triplicate samples. Data are representative of 3 similar experiments conducted on 2 donors.