American Society of Hematology

Figure 4.

$Figure 4. NVP-LAQ824 causes G1 arrest and apoptosis (increased sub-G1) in HMCLs and patient myeloma cells. (A) Freshly isolated patient cells were incubated with NVP-LAQ824 (0.01-1 μM) and examined for cell cycle profile using propidium iodide. There was a concentration-related increase in the sub-G1 fraction of patient cells, representing apoptosis. (B-C) MM.1S cells were cultured with 1 μM NVP-LAQ824 for 8 hours and 12 hours, washed twice, and then incubated for 24 hours prior to cell cycle examination by propidium iodide staining. The cell cycle changes shown in panel B are graphically represented in panel C. G1 arrest was observed at 8 hours, followed by increased sub-G1 (apoptosis) at 12 hours.$

NVP-LAQ824 causes G₁arrest and apoptosis (increased sub-G₁) in HMCLs and patient myeloma cells. (A) Freshly isolated patient cells were incubated with NVP-LAQ824 (0.01-1 μM) and examined for cell cycle profile using propidium iodide. There was a concentration-related increase in the sub-G₁ fraction of patient cells, representing apoptosis. (B-C) MM.1S cells were cultured with 1 μM NVP-LAQ824 for 8 hours and 12 hours, washed twice, and then incubated for 24 hours prior to cell cycle examination by propidium iodide staining. The cell cycle changes shown in panel B are graphically represented in panel C. G₁ arrest was observed at 8 hours, followed by increased sub-G₁ (apoptosis) at 12 hours.

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