American Society of Hematology

Figure 7.

$Figure 7. Stimulated secretion of WT and mutant rVWF. HEK293 cells were transiently transfected with WT rVWF or rVWF variants, and the quantity of VWF was measured by ELISA 2 days later with (+) or without (-) PMA stimulation. (A) Each point represents the amount of VWF secreted upon stimulation minus constitutive secretion at the indicated time. Error bars represent standard deviation (n = 3). (B) Each bar represents secreted VWF as a fraction of total VWF (secreted plus intracellular) ± standard deviation (n = 6). The numbers above the stimulated release bars (+) indicate the fold increase compared with the nonstimulated bars. Symbols (@ and*) indicate that these differences are statistically significant after Student t test (@ P < 1.10-5;* P < .02). &This difference is not statistically significant (P < .2).$

Stimulated secretion of WT and mutant rVWF. HEK293 cells were transiently transfected with WT rVWF or rVWF variants, and the quantity of VWF was measured by ELISA 2 days later with (+) or without (-) PMA stimulation. (A) Each point represents the amount of VWF secreted upon stimulation minus constitutive secretion at the indicated time. Error bars represent standard deviation (n = 3). (B) Each bar represents secreted VWF as a fraction of total VWF (secreted plus intracellular) ± standard deviation (n = 6). The numbers above the stimulated release bars (+) indicate the fold increase compared with the nonstimulated bars. Symbols (^@ and^*) indicate that these differences are statistically significant after Student t test (^@P < 1.10^-5;^*P < .02). ^&This difference is not statistically significant (P < .2).

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