American Society of Hematology

Figure 2.

$Figure 2. Stimulation of cultured γδ T cells rapidly induces an LN-homing program. (A) Change in chemokine receptor expression during stimulation of peripheral blood-derived γδ T-cell lines with IPP. Positivity for CCR7 (□), CCR4 (▵), and CXCR4 (○) was determined by flow cytometry as in Figure 1 and is expressed as the fraction (percent) of receptor-positive cells. (B) Chemotactic migration profile (±SD) of activated γδ T cells after 3 days of stimulation with IPP was examined as described in Figure 1. Asterisk points out that the migration response to LARC was examined with γδ T cells after 6 days instead of 3 days of stimulation, reflecting a delay in maximal CCR6 expression. One of 3 independent experiments is shown.$

Stimulation of cultured γδ T cells rapidly induces an LN-homing program. (A) Change in chemokine receptor expression during stimulation of peripheral blood-derived γδ T-cell lines with IPP. Positivity for CCR7 (□), CCR4 (▵), and CXCR4 (○) was determined by flow cytometry as in Figure 1 and is expressed as the fraction (percent) of receptor-positive cells. (B) Chemotactic migration profile (±SD) of activated γδ T cells after 3 days of stimulation with IPP was examined as described in Figure 1. Asterisk points out that the migration response to LARC was examined with γδ T cells after 6 days instead of 3 days of stimulation, reflecting a delay in maximal CCR6 expression. One of 3 independent experiments is shown.

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