nflammatory migration program in human peripheral blood-derived γδ T-cell lines. (A) Flow cytometric analysis of chemokine receptors (open histograms) on resting (previously activated, nonproliferating), IPP-selective γδ T-cell lines derived from human peripheral blood. Filled histograms represent control staining with isotype-matched Abs, and numbers refer to the percent receptor-positive cells. (B) Chemotactic migration and (C) Ca²⁺ mobilization responses in resting IPP-selective γδ T cells. Migration data, expressed as the fraction (percent) of input cells that have migrated (± SD), correspond to maximal responses determined by titration of chemokine concentrations; BCA-1 was only active at the highest concentration (3 μM) tested. Maximal chemotaxis responses were obtained with 1 or 10 nM I-TAC, SDF-1, MCP-1, RANTES, MDC, and MIP-1β; 100 nM eotaxin and LARC; 1 μM SLC; and 3 μM BCA-1. Ca²⁺ mobilization data correspond to 100 nM of chemokines. Functional data are representative of at least 3 independent experiments.