T-cell responses to EBV are partially preserved following allodepletion with haplo-identical LCLs. The figure shows the frequency of cells secreting IFN-γ as determined by ELISPOT assays. (A) Unmanipulated donor PBMCs (▦) or donor PBMCs allodepleted after stimulation with recipient LCLs from 3 different haplo-identical donor-recipient pairs (▪) were stimulated with irradiated autologous donor LCLs. (B) Unmanipulated donor PBMCs (▦) or donor PBMCs depleted with immunotoxin after stimulation with autologous LCLs (▪) were restimulated with irradiated autologous LCLs. Results are the mean number of specific spot-forming cells calculated by linear regression of duplicate wells assayed at 3 dilutions.