T-cell responses to adenoviral and CMV antigens are preserved after allodepletion. The figure shows the frequency of cells secreting IFN-γ as determined by ELISPOT assays. Unmanipulated PBMCs from 5 different seropositive donors (▦) or donor PBMCs allodepleted after stimulation with HLA-mismatched LCLs (▪) were stimulated with irradiated autologous PBMCs transduced with an adenoviral vector carrying the GFP gene (A), vaccinia vectors carrying the GFP gene with (B) or without (not shown) the CMV pp65 gene, or an adenoviral vector carrying the CMV pp65 and GFP genes (C). Results are shown numerically above columns. *The result falls above the axis limit. Results are the mean number of specific spot-forming cells calculated by linear regression of duplicate wells assayed at 3 dilutions.