![Figure 2. Proliferation and IFNγ production by Vγ9Vδ2 cells from cancer patients before and after treatment with zoledronic acid. PBMCs were cultured at 37°C, in the presence of 5% CO2, at 106/mL in 96-well flat-bottomed plates (0.2 mL/well), with isopentenylpyrophosphate (IPP; Sigma Chemical, St Louis, MO; 100 μM/mL final concentration) and 20 U/mL final concentration human recombinant IL-2 (▪) or with IL-2 alone as a control (□). Proliferation (A) was measured 7 days later by adding 1 μCi/well (0.037 MBq) [3H]thymidine (Amersham, Arlington Heights, IL) during the last 6 hours of culture. Cells were then harvested and [3H]thymidine incorporation was measured with a liquid scintillation β-counter. Results are expressed as mean counts per minute (cpm) of triplicate wells ± standard deviation (SD). IFNγ levels in the 48-hour culture supernatants (B) were assessed by 2 monoclonal antibodies (mAbs) sandwich enzyme-linked immunosorbent assay (ELISA) assay following the manufacturer's recommendations (R&D Systems, Minneapolis, MN).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/6/10.1182_blood-2003-05-1655/6/h81835050002.jpeg?Expires=1724263946&Signature=ENe7wOQ3py37DuvI-Yoq3a~u~2ftkpP7GP5f3Ulmou~nbPrjM~XG7tceY~Cx4a~14WivJ5-9-1w4Lm8XSUctEv7ai5PAN2fLnQS92HcZk7QZZlof3GZ6mNET8avRIjpgqNU99yT6MEtvEqxWlJKP5~CDSQVbCU93SBvBl6E44K-HasNj79qkKp51Ee2dfLaYzlHG~btm7RbEsz~tfTE7-mVIkYuMG8FwD59xG80EXWAgoURmY4DbkQpuI7sTTXtMLjzLAx8IfMGoc5PogjvoZVrNE9~qFo8FSkrPqpvtp~6Is840v~~abh1oyatJPBSQ01Vg71evO1YuNbkmAoNGHg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Proliferation and IFNγ production by Vγ9Vδ2 cells from cancer patients before and after treatment with zoledronic acid. PBMCs were cultured at 37°C, in the presence of 5% CO2, at 106/mL in 96-well flat-bottomed plates (0.2 mL/well), with isopentenylpyrophosphate (IPP; Sigma Chemical, St Louis, MO; 100 μM/mL final concentration) and 20 U/mL final concentration human recombinant IL-2 (▪) or with IL-2 alone as a control (□). Proliferation (A) was measured 7 days later by adding 1 μCi/well (0.037 MBq) [3H]thymidine (Amersham, Arlington Heights, IL) during the last 6 hours of culture. Cells were then harvested and [3H]thymidine incorporation was measured with a liquid scintillation β-counter. Results are expressed as mean counts per minute (cpm) of triplicate wells ± standard deviation (SD). IFNγ levels in the 48-hour culture supernatants (B) were assessed by 2 monoclonal antibodies (mAbs) sandwich enzyme-linked immunosorbent assay (ELISA) assay following the manufacturer's recommendations (R&D Systems, Minneapolis, MN).
