HK-1 and SubP expression in various subsets of thymocytes. Three CD44/CD25 sortings of DN cells and 2 CD4/CD8 sortings of total thymocytes were performed. RNA was prepared from sorted cells and reverse transcribed. Two rounds of nested PCR were performed, and the products were then detected with corresponding cDNA probes. Results from a representative set of sorted thymocytes are presented.