FACS analysis of cells recovered from FTOCs under various conditions. (A) CD4/CD8 staining of total thymocytes. The number at the top-right corner indicates the percentage of CD4⁺CD8⁺ cells. (B) CD25/CD44 staining of CD4^–CD8^– population. The number at the bottom right indicates the percentage of CD25⁺CD44^– DN3 cells.