CD4^dimCD8^bright T cells produce elevated levels of intracellular IL-4. (A-B) Type 1 (IL-2, IFNγ) and type 2 (IL-4) intracellular cytokines were evaluated by gating on CD4^dimCD8^bright (▪) and CD8⁺CD4^– (□) cells after SEB stimulation (A) or anti-CD3/CD28 (B). Data represent the mean percentage of cytokine expression ± SD of 3 healthy donors. (C) Intracellular cytokine staining of CD4^dimCD8^bright and CD8⁺CD4^– T cells generated after SEB stimulation, as well as SEB-stimulated CD4⁺ T cells, is shown. (D) CD8⁺ T cells were stimulated with SEB or anti-CD3/CD28, and intracellular perforin expression was evaluated by flow cytometry at day 6, when the CD4^dimCD8^bright phenotype is generated. Data represent the mean percentage of expression of perforin ± SD of 3 donors