Apoptotic profile of CD4^dimCD8^bright T cells. CD8⁺ T cells were stimulated with SEB, and the Tunel assay was performed using DNase-treated cultures as a positive control and TdT-negative cultures as a negative control. Percentage of Tunel⁺ cells was determined for CD4^dimCD8^bright T cells (▦) and CD8⁺CD4^– cells (▪) exposed to the same stimulation conditions as those generating the CD4^dimCD8^bright phenotype. Data represent the mean percentage of tunnel expression of 3 healthy donors, and the error bars represent ± SD. (B) Flow cytometric representation of experiment shown in panel A.