TCR and CD8 molecule expression on CD4^dimCD8^bright T cells. CD4^dimCD8^bright cells were generated by SEB stimulation of highly purified CD8⁺ T cells from healthy donors. (A) Representative level of CD4^dimCD8^bright expression, as indicated by the boxed gate in quadrant 2, is shown. (B) SEB-stimulated CD8⁺ T cells were stained on day 6 for surface expression of TCRαβ. The open histogram represents the isotype control and the filled histogram represents TCRαβ cells. Expression of CD8αβ is shown in panel C. Data are representative of 3 healthy donors.