Cell chemotaxis to supernatants from untreated and TGF-β 1–treated MS-5 cells. (Left) NCI-H929 cells were preincubated with anti-CXCR4 antibodies, Ptx, or adhesion medium alone and were allowed to migrate to lower chambers containing supernatants from MS-5 cells treated with or without TGF-β 1 (+ TGF-β 1 and –TGF-β 1, respectively). To some conditions, TGF-β 1 or SDF-1α was added to the medium (MS-5 Sup/+ TGF-β 1 or MS-5 Sup/+ SDF-1α, respectively). (Right) Mo7e cells were subjected to chemotaxis to supernatants from MS-5 cells incubated in the absence (–) or in the presence (+) of TGF-β 1. Also shown is cell chemotaxis to recombinant SDF-1α (100 ng/mL) compared with the chemotaxis to adhesion medium (control). Data represent the means ± SDs of duplicate samples from a representative result of 4 (left) and 3 (right) experiments. Reduction in chemotaxis was significant (**P < .005), according to Student 2-tailed t test.