Figure 1.
![Figure 1. Fluorescent genescan detection of FLT3-ITD. (A) Sensitivity of detection from cDNA (left column) versus DNA (right column). PCR products from a representative case (unique patient number [UPN] 529), after dilution into PBL DNA/RNA. The size of the ITD is given relative to the wild-type allele and the fluorescent scale indicated. (B) Subclone detection: minor subclones were more frequently detected from cDNA, as demonstrated for 2 representative patients. (C) Differences in relative intensity of the wild-type compared with FLT3-ITD alleles for cDNA versus DNA in a representative AML.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/6/10.1182_blood-2003-01-0162/6/h81834923001.jpeg?Expires=1725153405&Signature=nGcdxGe23M2ZJZz59q~bSNPfQiOmw~SjglAmwxpFWuWO9hrQ7ngHsgMfE8IzLeLr1oh1MNYp9dvlr~Wn4jBV2SEvlR12lN7b1XSJfGgWCqpKvDLyTc7rDaJsi5XjdRlacAebtko8nRirVIYv7Kr19QZGbEUFF-URL5OXgI7qr0nmo4DI7Gbz0uaesSx-VAy6rx0fl4rSkwCb3T1MTIPv7Gxz1OtA6SkdU7QJgh9a-E6N-M2xFc~q1Y7WcAshjr2oA1y1nQ96~rG7OuiG8wiKoXInM2OTIt93~0Dlu5d1-8veGoiYQ3HqriuSOUL~E4MKN6AqEews~qEkDEANp6PB4A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Fluorescent genescan detection of FLT3-ITD. (A) Sensitivity of detection from cDNA (left column) versus DNA (right column). PCR products from a representative case (unique patient number [UPN] 529), after dilution into PBL DNA/RNA. The size of the ITD is given relative to the wild-type allele and the fluorescent scale indicated. (B) Subclone detection: minor subclones were more frequently detected from cDNA, as demonstrated for 2 representative patients. (C) Differences in relative intensity of the wild-type compared with FLT3-ITD alleles for cDNA versus DNA in a representative AML.
