Surface phenotype of HF-derived leukocytes. (A-B) Cells harvested from 5-week-old HF cultures by vigorous pipetting were subjected to 3-color FACS analyses for CD45, c-kit, and Sca-1 (A) and for Lin, c-kit, and Sca-1 (B). (C) Cells harvested from 3-week-old HF cultures were stained for Lin, c-kit, and Sca-1. The data indicate the expression profiles for Lin and Sca-1 in nongated populations (left) and for Sca-1 and c-kit in the Lin^– populations (right). The results shown in this figure are representative of 5 (A-B) or 3 independent experiments (C).