Peptide presentation and induction of T-cell IFN-γ secretion by immature and mature MoDCs and CD1c⁺ PBDCs. Immature MoDCs (GM-CSF plus IL-4) or freshly sorted CD1c⁺ PBDCs were stimulated for 18 hours with the indicated stimuli, washed, pulsed with the indicated peptides, and used as stimulators (1 × 10⁴) of peptide-specific CTL lines as described in “Materials and methods.” T cells were assessed for intracellular IFN-γ secretion by flow cytometry. (A) NY-ESO-1b_157-165 presentation to NY-ESO-1b–specific CTL line. (B) EBV BMLF-1_280-288 presentation to EBV-specific CTL line. Data are presented as the percentage of all CD8⁺ T cells positive for intracellular IFN-γ staining. Data are representative of 3 separate donors.