Surface marker expression of freshly isolated and matured CD1c⁺ PBDCs. (A) Surface antigen expression of maturation markers on CD1c⁺ PBDCs. Isotype control is marked by the broken-lined, unfilled histogram; surface staining of PBDCs is shown by filled histograms. (B) Surface expression of the chemokine receptor CCR6, BDCA-3, and MMR. Crosshairs reflect background settings based on the isotype-matched Ab controls. Data are representative of 3 separate experiments. (C) Surface expression of CD83 on CD1c⁺ PBDCs and MoDCs at 2, 12, and 18 hours following stimulation either with GM-CSF and IL-4 or with GM-CSF and IL-4 in combination with TNF-α, IFN-α, and PGE₂ or with CD40L.