Morphology of immature and mature MoDCs and CD1c⁺ PBDCs. PBDCs were purified by negative depletion from the peripheral blood of patients treated with FL for 14 consecutive days and then FACS sorted to high purity (greater than 95%) on the basis of CD1c and HLA-DR expression. Autologous MoDCs were generated from blood taken prior to FL administration and cultured for 7 days prior to the parallel isolation of PBDCs. MoDCs were prepared by culturing purified CD14⁺ monocytes for 7 days in GM-CSF and IL-4. (A) Immature MoDCs. (B) Freshly sorted CD1c⁺ PBDCs. (C) MoDCs stimulated (second day) with CD40L. (D) CD1c⁺ PBDCs stimulated (second day) with CD40L. Figures are representative of more than 10 experiments. All photomicrographs are × 100 original magnification.