BAALC mRNA expression in pretreatment AML samples as determined by comparative RT-PCR and real-time RT-PCR.The gel illustrates the comparative RT-PCR. The values shown below (expressed as 2^μ(ΔCT); see “Patients and methods”) are from real-time RT-PCR experiments on samples from the same individuals. AML samples 1 to 3 represent cases with high level of BAALC transcripts. Samples 4 to 6 represent AML cases from the low BAALC group. M indicates size marker; R, reagent control.