![Figure 5. Effect of CD2 engagement on DC cytokine production and endocytic activity. (A-B) Freshly isolated pDCs (A) and GM-CSF– and IL-4–differentiated MDDCs (B) were cultured in the presence (▴ [IL-12] and ▪ [IL-1β]) or absence (♦ [IL-12] and * [IL-1β]) of anti-CD2 (T-111 and T-112) mAbs for 24 hours. The supernatants were assayed for IL-1β and IL-12 by ELISA, and results are typical of 3 similar experiments. (C) Endocytic activity of immature (▪) and mature (□) MDDCs was assessed. MDDCs, previously cultured for 24 hours in the absence or presence of either LPS or anti-CD2, were cultured with FITC-dextran for 30 minutes, and the amount of probe internalized was measured by FACS analysis. Expression indices for samples were determined, and data are presented as mean ± SEM. Findings were representative of 3 similar experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/5/10.1182_blood-2002-07-2206/6/h81734838005.jpeg?Expires=1725025194&Signature=CAIeXtGoctiv8uF14cADXgMAXSKfQJ9xcA76bzrLA65LtX~Rt6mfKeNlyPw~6gbVmgoqY6rmRqUWPEA-WBqHL~E5Es8Y83pRA0yNyjz~MOXPs6VRVYVju3MyPwG3t2~Nsvzq9chBXKSJ4tCBsynOi63jGkvcqCm-OiPduk33G1EVMsx~J84AOECrnXp0p7psWHqtvAA78pJXtly1QbpuT-FNruxEijaTlIUy3zDaQr83XvBaqGscnNwnbaEAQqSUrpTS~ygT-K0wYK7HMWO1ThMTEDPc14ZBxPywi1X408FNuTzNUrHMcu8Jt0jsxMtxw0E2CJmGAS~lCGcaLw8iKQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of CD2 engagement on DC cytokine production and endocytic activity. (A-B) Freshly isolated pDCs (A) and GM-CSF– and IL-4–differentiated MDDCs (B) were cultured in the presence (▴ [IL-12] and ▪ [IL-1β]) or absence (♦ [IL-12] and * [IL-1β]) of anti-CD2 (T-111 and T-112) mAbs for 24 hours. The supernatants were assayed for IL-1β and IL-12 by ELISA, and results are typical of 3 similar experiments. (C) Endocytic activity of immature (▪) and mature (□) MDDCs was assessed. MDDCs, previously cultured for 24 hours in the absence or presence of either LPS or anti-CD2, were cultured with FITC-dextran for 30 minutes, and the amount of probe internalized was measured by FACS analysis. Expression indices for samples were determined, and data are presented as mean ± SEM. Findings were representative of 3 similar experiments.
