Phenotypic characteristics of mature pDCs, pMo's, and MDDCs. CD14⁺ Mx's were cultured in 10% PHS for 5 days, separated into mature pDCs and pMo's, and analyzed by flow cytometry for MHC class II, costimulatory, and adhesion molecules (A). Analysis of the same surface antigens on GM-CSF/IL-4—generated MDDCs was also performed (B). The pDCs and pMo's were recultured for 24 hours in fresh media, and surface expression of the above molecules was reassessed (C). Dot plots were divided into upper and lower regions, which represent high and low MFI populations. Quadrant boundaries were set using isotype control. Percentages indicate the percentage of cells within the gated region. Values within parentheses represent the MFI of the high or low populations. Results are representative of data from more than 3 experiments.