TNF-α and IFN-γ intracytoplasmic expression in BM MNCs in individual FA patients. CD3 phenotypically defined MNCs were chosen because they contain T cells that in healthy individuals and in other marrow failure diseases such as AA^10-12  are known to elaborate the tested cytokines. TNF-α and IFN-γ expression are provided for each patient, both as percentage of positive cells and as MRFI, in each analyzed marrow cellular subset (▪ CD3⁺ cells, ▨ CD3^– cells). (A) TNF-α intracytoplasmic expression shown as percentage of cells in the cellular subsets (CD3⁺, CD3^– cells) expressing this cytokine. (B) TNF-α intracytoplasmic expression shown as MRFI of cells in the cellular subsets (CD3⁺, CD3^– cells) expressing this cytokine. (C) IFN-γ intracytoplasmic expression shown as percentage of cells in the cellular subsets (CD3⁺, CD3^– cells) expressing this cytokine. (D) IFN-γ intracytoplasmic expression shown as MRFI of cells in the cellular subsets (CD3⁺, CD3^– cells) expressing this cytokine.