Figure 10.
![Figure 10. TAT-NBD inhibited basal and LPS-induced IKK activity in a dose-dependent manner. The effect of TAT-NBD on constitutive and LPS-induced IKK activity was studied with in vitro kinase assay. Cells (6 × 106) pretreated with either buffer control, TAT-NBD (NBD), or the mutant control (MUT) were stimulated with 1 μg/mL LPS or left untreated. Whole cell lysates were immunoprecipitated with a monoclonal IKKα antibody. Precipitates were incubated with 1 μg GST-IκBα and 3 μCi (0.111 MBq) [γ-32]ATP. Samples were boiled and subjected to 12% SDS-PAGE and autoradiography. Equal loading was confirmed by IKKα Western blot.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/6/10.1182_blood-2002-09-2960/6/h81834919010.jpeg?Expires=1724244728&Signature=Boq3dkI9agTQyqXAWhvrGB4DACXS~OaK3vvEsVEAKunmxafcRv4rGDTlNhNuEH-keOMUbTfkge6J9VkG9Z3mAabtrXFBI6xhFFgnLPekMHRWJmOJ0Q-7~LPLkGcChM~-9mZqK1GyNLekqJnh25c7R7tjGJsEoR2UYeigku6Ve2hRLT4axh0hI9GSoBi-cwxD-OJJJhVGInoeUygEmb57fWbhFsBgAGD5l2eS5r5HpX0gzlgk3nB4eLphw5NuirhixG6-9nnDsiSRDf~ogl-kl4YGyMXspJGhgtKcAnJgZfc1~Lgq-Gx9iEq4-Zgz4d3aPyT0dAFaRqicjZuY92ErfA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
TAT-NBD inhibited basal and LPS-induced IKK activity in a dose-dependent manner. The effect of TAT-NBD on constitutive and LPS-induced IKK activity was studied with in vitro kinase assay. Cells (6 × 106) pretreated with either buffer control, TAT-NBD (NBD), or the mutant control (MUT) were stimulated with 1 μg/mL LPS or left untreated. Whole cell lysates were immunoprecipitated with a monoclonal IKKα antibody. Precipitates were incubated with 1 μg GST-IκBα and 3 μCi (0.111 MBq) [γ-32]ATP. Samples were boiled and subjected to 12% SDS-PAGE and autoradiography. Equal loading was confirmed by IKKα Western blot.
