PCR and Southern blot of lentiviral vector from dog tissues. PCR of 500 ng genomic DNA was used to detect the lentiviral vector from a variety of tissues in the treated dog (A). The integrity of tissue genomic DNA was confirmed by PCR amplification of the canine β-actin gene (B). After visualization of the ethidium bromide stained bands, the same agarose gel was used to perform Southern blot analysis (C).