Inhibition of HO-1 induction in RAECs. (A) Effect of pretreatment of HbLDL by ebselen and HO-1 enzyme. (B) Effect of pretreatment of HbLDL by ebselen and NaCN. HbLDL samples were pretreated with ebselen or HO-1 enzyme or NaCN to respectively reduce LOOH and degrade/inhibit heme. The LDL was then added to cells for 24 hours and HO-1 levels were analyzed as described. Details of pretreatment conditions are provided in “Materials and methods.”