Figure 4.
![Figure 4. Microsatellite instability in CFUs isolated from mice receiving MSH2–/– bone marrow. Individual CFUs were harvested from mice receiving transplants of 100% MSH2–/– cells and subjected to PCR using a 33P-labeled primer specific for the informative locus D3mit 203. PCR products were electrophoresed on a 7% denaturing gel. The representative gel depicts characteristic microsatellite instability in 2 of 13 CFUs (lanes 3 [insertion] and 12 [deletion]) from secondary recipient mice. Similar evaluations of wild-type marrow from primary and secondary recipients failed to show evidence of microsatellite instability.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/5/10.1182_blood-2002-10-3035/6/h81734841004.jpeg?Expires=1725025794&Signature=LxRYIhLYFD5Fxclolbk776G8Axhj-PquWVrWmrV~RqEpJ33ycpkRUlK0ua25iYmvQEjr8hd9vMPu5wu9eTHDiKulOVJKIA9dzCYI-Lxvc7KHM2tgRS7CEDD2K9866EciZgdvIj9FLGbHqzK7dERCf8NPVZzDQyWZ~NcsTu8UfHFRXIHFHVsakV0tXNXkRddibGSP5Y7WiIOZCWMakWP0JrNlpM5M-PwzCYk7nhbns295jX7vfD02-VNkFFNQS6ADBWLR7XikAi6SKJ5pDs5IsWPAOF-dWwKEvyfTq7edtHmUpzId19cQ8~uPXmGnoHYLBBkmU0uzjOGIQx03Ug1Yig__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Microsatellite instability in CFUs isolated from mice receiving MSH2–/– bone marrow. Individual CFUs were harvested from mice receiving transplants of 100% MSH2–/– cells and subjected to PCR using a 33P-labeled primer specific for the informative locus D3mit 203. PCR products were electrophoresed on a 7% denaturing gel. The representative gel depicts characteristic microsatellite instability in 2 of 13 CFUs (lanes 3 [insertion] and 12 [deletion]) from secondary recipient mice. Similar evaluations of wild-type marrow from primary and secondary recipients failed to show evidence of microsatellite instability.
