Cell cycle distribution among lineage-negative cells recovered from P1 and P2 marrow transplant recipient mice. Bone marrow MNCs were stained with PE-conjugated lineage-specific antibodies (“Materials and methods”) followed by Hoechst dye. The percentage of lineage-negative cells in the S-G2-M phase of the cells cycle was identified. (A) P1 mice that received transplants of either 100% MSH2–/– (□) or 100% wild-type (▪) marrow. Three weeks after transplantation, mice were treated with TMZ or left untreated. Mice were killed at 24 hours or 1 week after treatment. (B) Marrow cells collected from P1 and P2 serial transplant recipients. P1 mice received transplants of a mixture of 10% MSH2–/– and 90% wild-type cells and were treated with TMZ or left untreated. Marrow cells were harvested from P1 and P2 recipient mice 8 weeks after transplantation. (C) Representative histograms comparing the percentage of S-G2-M populations from wild-type and MSH2–/– mice.
