The allogeneic response of TEa Tg T cells to DST and skin is temporally and spatially separated. CFSE-labeled Tg TEa T cells were adoptively transferred into RAG^–/– recipients together with CB6F₁ DST, CB6F₁ skin, or syngeneic skin as a negative control for proliferation. At days 4 and 8, cells from draining and nondraining LNs were harvested and CFSE dilution of the TEa Tg T cells was analyzed by flow cytometry. Total number of TEa Tg T cells/LNs is shown for each group.