Figure 7.
No defect in self-renewal in stem cells from WASP-deficient mice. Long-term bone marrow cultures were established on MS-5 feeder layer with bone marrow cells from wild-type and WASP-deficient mice. First, 3 × 106 bone marrow cells were seeded into 10 mL culture medium in a 25-cm2 flask. Half of each culture was removed every week and replaced with fresh medium. A flask of each culture (one from wild-type and one from WASP-deficient cells) was stopped every week. Nucleated cells were counted and a progenitor assay was performed. No differences were found between the numbers of nucleated cells or clonogenic progenitors of wild-type and WASP-deficient cultures.
