Derivation of the JSB3 cell line. (A) Derivation of JSB3 cells. One-color histograms of HECA-452 (filled) or isotype control (open) staining on parental Jurkat cells (left), JS9-78 cells (center), or JSB3 cells (right) treated for 2 days with either 10 nM PMA (Jurkat and JS9-78) or 1 nM PMA (JSB3). Sort gates for sorting of PMA-treated JS9-78 are indicated. JSB3 cells stain at higher levels than JS9-78 for HECA-452; Jurkat cells show little or no HECA-452 staining. (B) RT-PCR shows much higher levels of FucT-VII mRNA in PMA-treated JSB3 cells than in parental Jurkat cells. Titered amounts (2-fold dilutions) of the input cDNA are indicated from left to right. (C) Higher and (D) more sustained levels of HECA-452 staining are observed in PMA-treated JSB3 cells than in PMA-treated JS9-78 cells. The percent HECA-452⁺ is determined by comparison with a negative control.