Schematic representation of p97 regulation of plasminogen and cell migration. This schematic representation summarizes the results obtained for p97 in the present study. (A) The interaction of pro-uPA and plasminogen with soluble activity increases the activation of plasminogen. This induction could be inhibited by the mAb L235, which recognizes a conformational epitope on p97. (B) The addition of mAb L235 reduces the plasminolytic activity on HMEC-1 cell surface and results in an inhibition of cell migration. (C) The interaction of plasminogen and pro-uPA with membrane-bound p97 (mb p97) is diminished when exogenous, competing human recombinant p97 is added. This also caused a decrease in the activation of plasminogen (plg) and leads to an inhibition of cell migration. This representation of the interaction between p97 and pro-uPA indicates that the balance between membrane bound and soluble p97 may be crucial for cell migration.