Figure 2.
![Figure 2. Biospecific interaction analysis in real time between p97 and various anti-p97 mAbs. p97 was immobilized on a sensor chip (CM5) using standard coupling procedures incorporating NHS, EDC, and ethanolamine. Different mAbs directed against p97 (HybC, HybE, HybF, L235, 2C7, 9B6), diluted to 0.05 μg/μL in Ringer-HEPES, were injected into the BIAcore at a flow rate of 5 μL/min. The surface plasmon resonance response obtained for these mAbs was plotted (in relative units [RU]) as a function of time. After each injection, immobilized p97 was regenerated with 0.2M glycine at pH 2 for 2 minutes followed by a 2-minute injection of ferric ammonium citrate (1 mM) (n = 4).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/5/10.1182_blood-2003-01-0166/6/h81734874002.jpeg?Expires=1724297034&Signature=mK2id14nqb3A6rmvS76k7HPZ6vyHOn7Emv0frmWLfkswrO5Zz18UVRCA7ChU8JDH3NJb7i1wW2mwXQyDM3JULed06g3ZAOXHq3D5TXc~4J6SGxoPR-2Ta8SrvI1dWLv0PM8h6jURpEDduOKYvr7ptM7J~Ii8J244wn6RBg~BB6cL6O9~5h6lrimMfVHHzbP7ZimiJT-QsdGCpth4pzH1TXlL-1zh4XbQQf6LdT3LMmLNH~z8haB9O89uUdILRGKWMha2zh1BA5yxT7IrhKc70CguOtoWiFARF7ulKYrSObzDStT3NbybcwrlnLb5TxDI3-My23IRHSjUgsSIU~P53g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Biospecific interaction analysis in real time between p97 and various anti-p97 mAbs. p97 was immobilized on a sensor chip (CM5) using standard coupling procedures incorporating NHS, EDC, and ethanolamine. Different mAbs directed against p97 (HybC, HybE, HybF, L235, 2C7, 9B6), diluted to 0.05 μg/μL in Ringer-HEPES, were injected into the BIAcore at a flow rate of 5 μL/min. The surface plasmon resonance response obtained for these mAbs was plotted (in relative units [RU]) as a function of time. After each injection, immobilized p97 was regenerated with 0.2M glycine at pH 2 for 2 minutes followed by a 2-minute injection of ferric ammonium citrate (1 mM) (n = 4).
