Figure 1.
![Figure 1. Transcytosis of p97 across BBCEC monolayers. (A) Transcytosis experiments were performed at 37°C for 2 hours. [125I]-p97 (25 nM) was added to the upper side of the cell-covered filter in the absence or presence of RAP (650 nM) or BSA (5 μM). At the end of the experiment, radiolabeled proteins were measured in the lower chamber of each well by TCA precipitation. Results represent means ± SE (n = 6). (B) p97 was immobilized on a sensor chip surface (CM5) as described in “Materials and methods.” p97, RAP, and BSA (5 μg/100 μL) were injected over immobilized p97. One representative experiment is shown (n = 3).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/5/10.1182_blood-2003-01-0166/6/h81734874001.jpeg?Expires=1724297748&Signature=LW4Ws1HNW2byR9RmYP3w3uPa7i4gimLB94w3iZuw22uBpwWIm4N5kvnWG0dqmjYf~~XRdZ77~tDfbih34yEOZKpuA3vJMXXEwSrWhJFq5l5aIXqR7LeXRfNrwVT~P8kZg5T~s3LRDCnX24HFTqE9i4QvwBtAGREsFV5ySQZ92H~5JpKbJKUq-nHYa4TJTVMHRK9fc~7g0Uq7pFA~IFeXSwiHa3476Eg1~A8xH8nyeZtukFbY5mVXILMGwejZsKdfNGdKWmxojIWEPOaxef8L-eO-9ee0DVuxsQ4dhkBBL3DpVEGY2tC8~NTm~B4HEzn9T6ESJG2whPvKZw2~dliBkQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Transcytosis of p97 across BBCEC monolayers. (A) Transcytosis experiments were performed at 37°C for 2 hours. [125I]-p97 (25 nM) was added to the upper side of the cell-covered filter in the absence or presence of RAP (650 nM) or BSA (5 μM). At the end of the experiment, radiolabeled proteins were measured in the lower chamber of each well by TCA precipitation. Results represent means ± SE (n = 6). (B) p97 was immobilized on a sensor chip surface (CM5) as described in “Materials and methods.” p97, RAP, and BSA (5 μg/100 μL) were injected over immobilized p97. One representative experiment is shown (n = 3).
