MP inhibitors block de-adhesion of THP-1 cells bound to CX3CL1–ECV-304 cells. WT and CX3CL1–ECV-304 cells were loaded with fluorescently labeled THP-1 cells. After removal of nonadherent cells by gentle washing, the fluorescence signal of the adherent cells was determined. Subsequently, cells were washed under agitation in the presence or absence of different concentrations of the mixed ADAM10/TACE inhibitor GW280264X or the selective ADAM10 inhibitor GI254023X. After 15 minutes, the detached cells were washed off and the fluorescence signal of the cells that remained adherent was quantified. Thereafter, an additional wash was performed for 15 minutes and fluorescence was read (mean and SD, n = 4). Statistically significant preservation of cell adhesion in the presence of inhibitors compared with the control receiving no inhibitors is indicated by asterisks (P < .05). Results are presented as 1 representative of 3 independent experiments.