TGFβ blocks LPS-mediated stabilization of KC mRNA. (A) TG-elicited macrophages were stimulated with LPS (10 ng/mL) in the presence or absence of TGFβ (20 ng/mL) for 3 hours. Each culture was subsequently treated with ActD (5 μg/mL), and at the indicated times cultures were used to prepare total RNA for analysis of KC mRNA by Northern hybridization (B). The autoradiographs were quantified using the IMAGE software from the National Institutes of Health (NIH, Rockville, MD). Similar results were obtained in 2 separate experiments.