KC mRNA sequence confers sensitivity to TGFβ. RAW264.7 cell lines stably transfected with the indicated genomic or cDNA clones of KC were stimulated with LPS (10 ng/mL) in the presence or absence of TGFβ (20 ng/mL) for the indicated times. Total RNA was prepared and used to determine the levels of transgenic KC mRNA by Northern hybridization. Similar results were obtained in 3 separate experiments.