Flow cytometric analysis of CD54 and CD62P expression on HUVECs. In panel A, surface expression of CD54 (intercellular adhesion molecule-1) upon treatment with Stx1 or Stx2 for 4 hours and 24 hours is illustrated (dashed lines) in comparison with untreated control cells (solid lines). The same layout is used in panel B for presentation of flow cytometric data obtained with a CD62P (P-selectin)–specific antibody. The data shown represent 3 independent experiments.