Transactivation potential and induction of NE expression by p42 and mutant C/EPBα. (A) Transactivation potential of p42 and mutant C/EBPα in 293T cells transfected with the indicated expression plasmids and the wild-type or mutant G-CSFR reporter gene. Luciferase activities were measured 24 hours after transfection and graphically represented as the percentage of luciferase activity ± SEM relative to p42 C/EBPα assigned as 100% activity. Representative of 4 different experiments. Inset shows transactivation of the mutant reporter plasmid used as control. (B) Induction of NE expression (by RT-PCR) in GFP-sorted p42- or mutant C/EBPα-expressing 32Dcl3 cells. Total RNA was isolated from cells kept in culture for the indicated time in the presence of IL-3. hnRNP E1 transcripts were detected as a loading control.