Figure 6.
![Figure 6. Ca2+ responses following RP105 ligation. (A) Splenocytes from wild-type or CD19–/– mice were loaded with 1 μM indo-1-am ester and stained with FITC-labeled anti-B220 mAbs. B cells were examined for relative [Ca2+]i levels by flow cytometry after gating on the B220+ population of cells. (B-C) Wild-type B cells were pretreated with 1, 10, or 25 nM of Wortmannin for 10 minutes (B), and wild-type and CD19–/– B cells were pretreated with 15 μM of LY294002 for 10 minutes (C). Cells were stimulated by anti-RP105 Abs (5 μg/mL) as indicated by arrows. An increase in [Ca2+]i over time is shown as an increase in the ratio of indo-1 fluorescence. Results represent those obtained in at least 3 experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/102/4/10.1182_blood-2002-11-3573/6/h81634769006.jpeg?Expires=1724239925&Signature=cRHmcJ3Ly1khxNs~O9f-eL1VK7A1AoT3YmJCo6du-8UZA9FLITo1BK429ejomCzrrz9MN~vQau-SV1AoPT3XMQypWOtRgjtPdi3A3ubqiYk-tMViDSiqgKQj9vQgtIj4yKI7yV0-jHtiC3p4OjYeLkARtsqzMygyb0yyZZAiWCE3i6zLRuxlwuSzdr6-UTcHZy2lniEWVCXL-K5Nz9oznRS6TpVnSVifyFdIE~1VbBs7kxKQqYGFrU6efL1Te5j9Kzb9x6RZwObB8bxeH7sQHm4XrZqKBFju~O18bHPTO79wZG0EOezmKTQPpuY3EkiTKdDSnBliY4JQuva~fyfBVA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Ca2+ responses following RP105 ligation. (A) Splenocytes from wild-type or CD19–/– mice were loaded with 1 μM indo-1-am ester and stained with FITC-labeled anti-B220 mAbs. B cells were examined for relative [Ca2+]i levels by flow cytometry after gating on the B220+ population of cells. (B-C) Wild-type B cells were pretreated with 1, 10, or 25 nM of Wortmannin for 10 minutes (B), and wild-type and CD19–/– B cells were pretreated with 15 μM of LY294002 for 10 minutes (C). Cells were stimulated by anti-RP105 Abs (5 μg/mL) as indicated by arrows. An increase in [Ca2+]i over time is shown as an increase in the ratio of indo-1 fluorescence. Results represent those obtained in at least 3 experiments.
