Expression and function of TLRs in transformed B cells. (A) Total RNA was isolated from transformed cell lines representing B cells at various stages of differentiation. RNA (7 μg) was subjected to Northern analysis employing a radiolabeled probe complementary to TLR9 (top) and TLR10 (middle). Ribosomal RNA was used as the loading control (bottom). The electrophoretic mobility of the 18S and 28S subunits of ribosomal RNA is indicated. These results are representative of 2 independent experiments. (B) DHL-4 and Karpas cell lines were seeded in a 24-well plate (0.5 × 109 cells/L) in complete RPMI. Cells were stimulated for 24 hours with CpG DNA (2006) (0.6 μg/mL). Supernatants were removed from cells and analyzed for chemokine production by ELISA. Results are expressed in pg/mL (mean ± SEM) and are representative of 2 independent experiments.
