Figure 8.
PLCγ2–/– platelets showed limited adhesion on collagen under static condition. (A) Washed platelets from wild-type (WT), PLCγ2–/–, and FcR γ-chain–/– were seeded on collagen-coated surfaces for 30 minutes. After unbound platelets were removed, the platelets were fixed, permeabilized, and stained by TRITC-labeled phalloidin for actin fibers and analyzed by fluorescent microscopy. (B) Mean number of adhered platelets ± SEM per 0.0364 mm2 from 14 different images from 2 experiments. **P < .01. Photo images are representative of 14 to 21 different images from 2 experiments.
