Figure 1.
PLCγ2–/– platelets exhibit a small increase in light transmission upon stimulation by collagen. (A) Heparinized PRP from wild-type, PLCγ2–/–, and FcRγ-chain–/– mice was stimulated with 10 μg/mL collagen for 5 minutes. (B) Washed platelets from wild-type and PLCγ2–/– mice were stimulated with 1 U/mL thrombin for 5 minutes. (C) Washed platelets from wild-type, PLCγ2–/–, and FcRγ-chain–/– mice were incubated without inhibitors (control) or with 20 μg/mL anti-α2 (HMα2), 10 μM lotrafiban, or a combination of 20 μg/mL HMα2 and 10 μM lotrafiban for 5 minutes. Platelets were stimulated with 10 μg/mL collagen for 5 minutes. The data are representative of 3 experiments.
