CALM-AF10. (Top) CALM-AF10 fusion gene. Wild-type AF10 functional domains are indicated and the cysteines of the extended LAP/PHD motif are detailed. CALM and AF10 exon nomenclatures are reference sequences NM007166 and NM004641, respectively. Nucleotide nomenclature is identical to that used previously.10 Identified FT points within CALM and AF10 are shown as vertical arrows with solid lines, alternative splice sites as vertical arrows with dotted lines. Position of RT-PCR (black) and sequencing (gray) primers are indicated. (Bottom) RT-PCR products using AF10 AS1002 (upper) or AF10 AS559 (lower) primers in CALM-AF10+ patients with IM or TCRγδ T-ALLs. Unique patient numbers (UPNs) are indicated above each lane. MWM indicates molecular weight markers.
