American Society of Hematology

Figure 6.

$Figure 6. Resveratrol induces apoptosis in AML cells. (A) OCIM2 cells were incubated in the absence (control) or presence of 5, 10, 25, 50, or 100 μM resveratrol. The fraction of cells undergoing apoptotic cell death was detected by annexin V–FITC. The percentages presented in each frame depict the dose-dependent increase in the apoptotic cell fraction. (B) AML cells were incubated with or without 25 μM resveratrol. Cytospun cells were stained with Wright stain and TUNEL. After exposure to resveratrol (Wright stain in right upper corner) several cytospun cells show larger cytoplasm and other cells show pyknotic nuclei. Dividing cells were not found (as shown in the control culture in left upper corner) and numerous yellow-appearing apoptotic cells were detected (right lower corner). Original magnification, × 10.$

Resveratrol induces apoptosis in AML cells. (A) OCIM2 cells were incubated in the absence (control) or presence of 5, 10, 25, 50, or 100 μM resveratrol. The fraction of cells undergoing apoptotic cell death was detected by annexin V–FITC. The percentages presented in each frame depict the dose-dependent increase in the apoptotic cell fraction. (B) AML cells were incubated with or without 25 μM resveratrol. Cytospun cells were stained with Wright stain and TUNEL. After exposure to resveratrol (Wright stain in right upper corner) several cytospun cells show larger cytoplasm and other cells show pyknotic nuclei. Dividing cells were not found (as shown in the control culture in left upper corner) and numerous yellow-appearing apoptotic cells were detected (right lower corner). Original magnification, × 10.

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