Effect of resveratrol on IL-1β (A) Effect of resveratrol on the production of IL-1β by OCIM2 cells. Cells were incubated in RPMI 1640 supplemented with 10% FCS in the presence or absence of 25 μM resveratrol (Res). The amount of mature IL-1β protein produced by these cells was then assessed by Western immunoblotting. The arrows point to the 17.5-kDa mature IL-1β protein and β actin. The first lane (control) shows commercially available, pure, mature IL-1β protein, the second lane shows protein from cells incubated in tissue culture medium, and the third lane shows protein from cells incubated with resveratrol. (B) Effect of resveratrol on membrane-bound IL-1β. OCIM2 cells were incubated in the presence and absence of resveratrol as described. The level of membrane-bound IL-1β was measured by flow cytometry; thin line denotes isotypic control; filled histogram, untreated cells; and thick line, cells treated with resveratrol.