Functional analysis of lymphoid cell populations following DII of Jak3^-/-. For T-cell proliferation, PB cells from control C57/BL6 mice (WT), Jak3^-/- mice (KO), DII-injected Jak3^-/- mice (KO + DII), or Jak3^-/- mice undergoing transplantation with ex vivo–infected BMCs (Ex Vivo) were plated in the presence of either media alone or with ConA plus IL-2, cultured for a total of 48 hours in vitro, pulsed with [³H]thymidine, and then harvested according to the procedures described in “Materials and methods.” Data are plotted as the means ± SDs counts per minute (cpm) of triplicate wells (A). Error bars indicate standard deviations. (B) For B-cell function, mice were challenged with chicken IgG, and 14 days after inoculation serum plasma was collected to measure for the presence of chicken IgG–specific antibodies (absorbance at 405 nm in ELISA assay according to the procedures described in “Materials and methods”).