IgE, but not SF, may require a calcium-dependent PKC to trigger BMMC adhesion to FN. (A) Adhesion of normal BMMCs to FN in response to 1 μg/mL IgE or 5 ng/mL SF in the presence of vehicle control (□), 1 μM U73122 (▪), or 1 μM U73343 (▨). Adhesion was significantly (P < .05) different between stimulated samples in the presence and absence of U73122 but not U73343. (B) Adhesion of BMMCs to FN in response to 1 μg/mL IgE or 5 ng/mL SF in the presence of the indicated concentrations of Compound 3 (C3). Adhesion was significantly (P < .05) different between stimulated samples in the presence and absence of C3 at 25 and 50 μM C3. (C) Adhesion of BMMCs to FN in response to 1 μg/mL IgE or 5 ng/mL SF in the presence of the indicated concentrations of Gö6976 (Gö). Adhesion was significantly (P < .05) different between IgE- but not SF-stimulated samples in the presence and absence of Gö. All adhesion assays were 30 minutes, and the results shown are the mean ± SEM of triplicate determinations. Background adhesions (A, 4%; B, 13%; and C, 4%) were subtracted. Similar results were obtained in 4 (A), 4 (B), and 3 (C) separate experiments.