Figure 4.
Figure 4. Sickle RBC adhesion to TSP is inhibited by the purified scFv TSP-A10. Washed sickle RBCs and TSP immobilized on perfusion chamber wells were incubated with control buffer (TSP), purified scFv TSP-A10 (10 μg/mL, n = 10), or purified control scFv (a-hTG; 10 μg/mL, n = 6) for 60 minutes at room temperature. Treated RBCs were perfused through flow chambers at a wall shear stress of 1 dyne/cm2, and adherent cells were counted as described in Figure 3. The results are shown as the means ± SEs of adherent RBCs/mm2. The * indicates a P value of .0002 or less compared with control scFv.

Sickle RBC adhesion to TSP is inhibited by the purified scFv TSP-A10. Washed sickle RBCs and TSP immobilized on perfusion chamber wells were incubated with control buffer (TSP), purified scFv TSP-A10 (10 μg/mL, n = 10), or purified control scFv (a-hTG; 10 μg/mL, n = 6) for 60 minutes at room temperature. Treated RBCs were perfused through flow chambers at a wall shear stress of 1 dyne/cm2, and adherent cells were counted as described in Figure 3. The results are shown as the means ± SEs of adherent RBCs/mm2. The * indicates a P value of .0002 or less compared with control scFv.

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